Application of glutamine metabolism-related gene signatures in the non-invasive diagnosis of coronary artery disease: integrating bulk and single-cell transcriptomics.
Z, Z., Y, L., J, L., Y, G., Z, Y., H, L., & Q, L. (2026). Application of glutamine metabolism-related gene signatures in the non-invasive diagnosis of coronary artery disease: integrating bulk and single-cell transcriptomics.. Journal of bioenergetics and biomembranes. https://doi.org/10.1007/s10863-026-10125-x
Z Z, Y L, J L, Y G, Z Y, H L, et al. Application of glutamine metabolism-related gene signatures in the non-invasive diagnosis of coronary artery disease: integrating bulk and single-cell transcriptomics.. Journal of bioenergetics and biomembranes. 2026; doi: 10.1007/s10863-026-10125-x
Z Z, Y L, J L, et al. Application of glutamine metabolism-related gene signatures in the non-invasive diagnosis of coronary artery disease: integrating bulk and single-cell transcriptomics.[J]. Journal of bioenergetics and biomembranes. 2026. DOI: 10.1007/s10863-026-10125-x.
@article{z2026,
author = {Zhao Z and Li Y and Li J and Gao Y and Yan Z and Li H and Liu Q},
title = {Application of glutamine metabolism-related gene signatures in the non-invasive diagnosis of coronary artery disease: integrating bulk and single-cell transcriptomics.},
journal = {Journal of bioenergetics and biomembranes},
year = {2026},
doi = {10.1007/s10863-026-10125-x},
note = {PMID: 42530694},
}
TY - JOUR AU - Zhao Z AU - Li Y AU - Li J AU - Gao Y AU - Yan Z AU - Li H AU - Liu Q TI - Application of glutamine metabolism-related gene signatures in the non-invasive diagnosis of coronary artery disease: integrating bulk and single-cell transcriptomics. T2 - Journal of bioenergetics and biomembranes PY - 2026 DO - 10.1007/s10863-026-10125-x AN - PMID:42530694 ER -
Coronary artery disease (CAD) is a common cardiovascular disorder strongly associated with glutamine metabolism. This study seeks to identify novel glutamine metabolism-related gene markers in CAD. Based on GEO datasets and glutamine metabolism-related genes, hub genes were pinpointed through WGCNA combined with LASSO, SVM-RFE, and random forest algorithms. Immune cell infiltration was estimated with the CIBERSORT algorithm. To confirm the expression and roles of hub genes, ox-LDL-stimulated HUVECs were analyzed in culture. MYBPC3 and TRIM47 emerged as candidate diagnostic signatures for CAD. Associations between MYBPC3/TRIM47 and immune cell infiltration were revealed. Upregulation of MYBPC3 and TRIM47 in CAD was confirmed in both GSE113079 and ox-LDL-treated HUVECs. Knockdown of TRIM47 or MYBPC3 promoted ox-LDL-induced proliferation of HUVECs, alleviated cellular apoptosis, and suppressed the expression of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) as well as M1 macrophage polarization. MYBPC3 and TRIM47 may serve as candidate diagnostic signatures for CAD.