Chronotherapy to reinforce circadian rhythms improves poststroke outcomes and glymphatic function in mice.
E, W., Y, Z., A, C., VS, V., E, N., MJ, G., E, D., E, B., W, S., & TM, A. (2026). Chronotherapy to reinforce circadian rhythms improves poststroke outcomes and glymphatic function in mice.. The Journal of clinical investigation. https://doi.org/10.1172/JCI201800
E W, Y Z, A C, VS V, E N, MJ G, et al. Chronotherapy to reinforce circadian rhythms improves poststroke outcomes and glymphatic function in mice.. The Journal of clinical investigation. 2026; doi: 10.1172/JCI201800
E W, Y Z, A C, et al. Chronotherapy to reinforce circadian rhythms improves poststroke outcomes and glymphatic function in mice.[J]. The Journal of clinical investigation. 2026. DOI: 10.1172/JCI201800.
@article{e2026,
author = {Waight E and Zhu Y and Caudell A and Vizcarra VS and Newbold E and Giannetto MJ and Duyvestyn E and Balbuena E and Song W and Arefin TM},
title = {Chronotherapy to reinforce circadian rhythms improves poststroke outcomes and glymphatic function in mice.},
journal = {The Journal of clinical investigation},
year = {2026},
doi = {10.1172/JCI201800},
note = {PMID: 42294892},
}
TY - JOUR AU - Waight E AU - Zhu Y AU - Caudell A AU - Vizcarra VS AU - Newbold E AU - Giannetto MJ AU - Duyvestyn E AU - Balbuena E AU - Song W AU - Arefin TM TI - Chronotherapy to reinforce circadian rhythms improves poststroke outcomes and glymphatic function in mice. T2 - The Journal of clinical investigation PY - 2026 DO - 10.1172/JCI201800 AN - PMID:42294892 ER -
Stroke remains a leading cause of morbidity and mortality worldwide, with few effective interventions to promote recovery. Targeting circadian timing and glymphatic function may represent viable therapeutic strategies. Here, we show that the small-molecule clock modulator, KL001; high-dose melatonin; acute light pulses; and active-phase time-restricted feeding were each sufficient to enhance glymphatic function in mice. Moreover, initiating treatment with either KL001 or active-phase time-restricted feeding 3 days after preclinical models of stroke improved motor outcomes, reduced lesion volume, increased glymphatic flow, and lowered poststroke brain cytokine burden. These findings suggest that reinforcing normal daily rhythmicity after stroke can markedly enhance neurological recovery, even when interventions are initiated several days after stroke onset.