Noninvasive whole-brain imaging of glymphatic dynamics.
N, W., X, Y., M, L., Q, L., AJ, C., P, H., L, D., S, D., B, M., & Y, X. (2026). Noninvasive whole-brain imaging of glymphatic dynamics.. Science advances. https://doi.org/10.1126/sciadv.aee4926
N W, X Y, M L, Q L, AJ C, P H, et al. Noninvasive whole-brain imaging of glymphatic dynamics.. Science advances. 2026; doi: 10.1126/sciadv.aee4926
N W, X Y, M L, et al. Noninvasive whole-brain imaging of glymphatic dynamics.[J]. Science advances. 2026. DOI: 10.1126/sciadv.aee4926.
@article{n2026,
author = {Wang N and Yu X and Lowerison M and Li Q and Canning AJ and He P and Dang L and Degan S and Mace B and Xu Y},
title = {Noninvasive whole-brain imaging of glymphatic dynamics.},
journal = {Science advances},
year = {2026},
doi = {10.1126/sciadv.aee4926},
note = {PMID: 42308292},
}
TY - JOUR AU - Wang N AU - Yu X AU - Lowerison M AU - Li Q AU - Canning AJ AU - He P AU - Dang L AU - Degan S AU - Mace B AU - Xu Y TI - Noninvasive whole-brain imaging of glymphatic dynamics. T2 - Science advances PY - 2026 DO - 10.1126/sciadv.aee4926 AN - PMID:42308292 ER -
Cerebrospinal fluid circulation through the glymphatic system plays a crucial role in removing metabolic waste from the central nervous system. However, the mechanism underlying the brain-wide glymphatic dynamics is not yet fully understood, in part due to the lack of glymphatic imaging technologies on deep brains. Here, we report a hybrid imaging technology that integrates three-dimensional photoacoustic tomography and ultrasound localization microscopy (3D-PAULM), enhanced by a photoacoustic dye with strong optical absorption in the second near-infrared window (NIR-II). 3D-PAULM allows for continuous, noninvasive, whole-brain imaging in mice through intact skull, providing superresolution mapping of the brain vasculature and highly sensitive tracing of the NIR-II dye in the glymphatic system. Using 3D-PAULM, we investigated the glymphatic function impaired by ischemic stroke, aging, and anesthesia. Our results provide insights into glymphatic transport under various physiological as well as pathological conditions and establish 3D-PAULM as a valuable tool for preclinical glymphatic research.