Mesenchymal Stem Cells of Epicardial Adipose Tissue Show Differences in Immunophenotype and Osteogenic Potential in Patients with Coronary and Non-Coronary Heart Disease.
OV, G., TA, S., EE, G., SE, D., YA, D., EV, F., AN, K., AB, N., & OL, B. (2026). Mesenchymal Stem Cells of Epicardial Adipose Tissue Show Differences in Immunophenotype and Osteogenic Potential in Patients with Coronary and Non-Coronary Heart Disease.. Cells. https://doi.org/10.3390/cells15141270
OV G, TA S, EE G, SE D, YA D, EV F, et al. Mesenchymal Stem Cells of Epicardial Adipose Tissue Show Differences in Immunophenotype and Osteogenic Potential in Patients with Coronary and Non-Coronary Heart Disease.. Cells. 2026; doi: 10.3390/cells15141270
OV G, TA S, EE G, et al. Mesenchymal Stem Cells of Epicardial Adipose Tissue Show Differences in Immunophenotype and Osteogenic Potential in Patients with Coronary and Non-Coronary Heart Disease.[J]. Cells. 2026. DOI: 10.3390/cells15141270.
@article{ov2026,
author = {Gruzdeva OV and Slesareva TA and Gorbatovskaya EE and Dolmatova SE and Dyleva YA and Fanaskova EV and Kokov AN and Nishonov AB and Barbarash OL},
title = {Mesenchymal Stem Cells of Epicardial Adipose Tissue Show Differences in Immunophenotype and Osteogenic Potential in Patients with Coronary and Non-Coronary Heart Disease.},
journal = {Cells},
year = {2026},
doi = {10.3390/cells15141270},
note = {PMID: 42505380},
}
TY - JOUR AU - Gruzdeva OV AU - Slesareva TA AU - Gorbatovskaya EE AU - Dolmatova SE AU - Dyleva YA AU - Fanaskova EV AU - Kokov AN AU - Nishonov AB AU - Barbarash OL TI - Mesenchymal Stem Cells of Epicardial Adipose Tissue Show Differences in Immunophenotype and Osteogenic Potential in Patients with Coronary and Non-Coronary Heart Disease. T2 - Cells PY - 2026 DO - 10.3390/cells15141270 AN - PMID:42505380 ER -
Coronary artery calcification (CAC) is a pressing issue in cardiology. Mesenchymal stem cells (MSCs) derived from epicardial adipose tissue (EAT) may serve as a source of osteoblasts in the cardiovascular system. This study aimed to evaluate and compare the immunophenotype, proliferation, and osteogenic potential of EAT-MSCs from patients with coronary artery disease (CAD) and those with aortic stenosis (AS). The immunophenotype of MSCs was analyzed based on key markers: CD105, CD90, CD73, CD31, CD34, CD45, HLA-DR. Cell proliferation was assessed by the doubling time of the population and the specific growth rate of the cultures. The osteogenic potential was evaluated by the expression levels of the RUNX2, SPP1, ALPL, and BGLAP genes using PCR, as well as the protein levels in supernatants via ELISA. Qualitative detection of osteogenic proteins in cells by immunofluorescence staining. The intensity of extracellular matrix mineralization was measured using photometric methods. CD73 expression in EAT-MSCs from CAD patients was nearly 50% lower than in EAT-MSC cultures from AS patients. Following osteogenic induction, EAT-MSCs from CAD patients showed increased expression of osteogenic marker genes and their corresponding proteins. The intensity of extracellular matrix mineralization by osteoblasts derived from EAT-MSCs of CAD patients was higher than in the comparison group. EAT-MSCs from CAD patients with coronary calcification demonstrated reduced CD73 expression in culture and enhanced osteogenic potential compared to patients without coronary artery involvement.