Tail Artery Blood Sampling in Isoflurane Anesthetized Rats Reduces Stress and Improves Reliability.

摘要

This method describes blood sampling via the tail artery in isoflurane-anesthetized rats. The use of isoflurane minimizes defensive movements, thereby enabling a controlled, low-stress procedure for both the animal and the researcher. Further advantages include the rapid recovery of animals after sampling due to the short-acting nature of isoflurane, the ability to collect comparatively large blood volumes from a single puncture site, and the suitability of the technique for repeated blood sampling. In addition, the rapid arterial blood flow may reduce the likelihood of coagulation artifacts, although this was not specifically evaluated in the present study. The results were determined as part of a pharmacokinetic study. Blood collection was successful in all animals, resulting in a 100% sampling success rate. No significant body weight loss was observed 24 h after blood sampling, and animals showed rapid recovery without visible signs of distress or procedure-related complications. Overall, this approach is highly suitable for experimental settings requiring reliable and reproducible blood collection with minimal handling stress. After a short learning phase, the procedure proved to be reliable for routine experimental use. However, the potential influence of isoflurane anesthesia on blood gas, metabolic, and endocrine parameters should be considered when planning experiments and interpreting the results.