Potassium-Selective Nanoelectrode Arrays for Single-Cell Profiling of Human iPSC-Derived Cardiomyocytes.
DP, M., R, B., A, C., E, S., K, M., H, V., S, R., I, A.D., A, T., & L, S. (2026). Potassium-Selective Nanoelectrode Arrays for Single-Cell Profiling of Human iPSC-Derived Cardiomyocytes.. ACS nano. https://doi.org/10.1021/acsnano.5c21944
DP M, R B, A C, E S, K M, H V, et al. Potassium-Selective Nanoelectrode Arrays for Single-Cell Profiling of Human iPSC-Derived Cardiomyocytes.. ACS nano. 2026; doi: 10.1021/acsnano.5c21944
DP M, R B, A C, et al. Potassium-Selective Nanoelectrode Arrays for Single-Cell Profiling of Human iPSC-Derived Cardiomyocytes.[J]. ACS nano. 2026. DOI: 10.1021/acsnano.5c21944.
@article{dp2026,
author = {Meganathan DP and Banzon R and Casanova A and Sarikhani E and Mahato K and Vu H and Reade S and Ambika Devarajan I and Tahir A and Sasi L},
title = {Potassium-Selective Nanoelectrode Arrays for Single-Cell Profiling of Human iPSC-Derived Cardiomyocytes.},
journal = {ACS nano},
year = {2026},
doi = {10.1021/acsnano.5c21944},
note = {PMID: 42611223},
}
TY - JOUR AU - Meganathan DP AU - Banzon R AU - Casanova A AU - Sarikhani E AU - Mahato K AU - Vu H AU - Reade S AU - Ambika Devarajan I AU - Tahir A AU - Sasi L TI - Potassium-Selective Nanoelectrode Arrays for Single-Cell Profiling of Human iPSC-Derived Cardiomyocytes. T2 - ACS nano PY - 2026 DO - 10.1021/acsnano.5c21944 AN - PMID:42611223 ER -
Potassium ion (K+) dynamics are central to cardiac electrophysiology, with early disruptions in K+ flux often preceding arrhythmia and contractile dysfunction. However, current sensing technologies, such as patch-clamp, microelectrode arrays (MEAs), and fluorescent indicators, either lack chemical specificity for K+ or are unsuitable for long-term, single-cell analysis. Conventional ion-selective electrodes (ISEs), while more selective, are limited by bulk-phase design and poor spatial resolution. To address these limitations, we present KINESIS (K+-Ion Nano-Electrode Selective Interface System), a nanofabricated platform that enables direct, label-free potentiometric measurement of K+ gradients with single-cell precision. KINESIS features high-aspect-ratio nanopillars coated with a valinomycin-based K+ recognition membrane, forming a stable, noninvasive interface with human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). This architecture allows localized, Nernstian sensing of K+ efflux or depletion without disrupting cell membranes. Pharmacological validation shows distinct potential shifts in response to caffeine and ouabain. KINESIS thus offers a highly selective, spatially resolved approach for studying K+ handling in cardiotoxicity screening and patient-specific disease modeling.