Experimental techniques to evaluate IA-MSCs-mediated alleviation of vasogenic edema following ischemic stroke.
A, D., N, M., G, K., A, B., D, S., V, K., A, D., U, M., A, K., & S, B. (2026). Experimental techniques to evaluate IA-MSCs-mediated alleviation of vasogenic edema following ischemic stroke.. Methods in enzymology. https://doi.org/10.1016/bs.mie.2026.06.021
A D, N M, G K, A B, D S, V K, et al. Experimental techniques to evaluate IA-MSCs-mediated alleviation of vasogenic edema following ischemic stroke.. Methods in enzymology. 2026; doi: 10.1016/bs.mie.2026.06.021
A D, N M, G K, et al. Experimental techniques to evaluate IA-MSCs-mediated alleviation of vasogenic edema following ischemic stroke.[J]. Methods in enzymology. 2026. DOI: 10.1016/bs.mie.2026.06.021.
@article{a2026,
author = {Datta A and Malik N and Karmarkar G and Barik A and Sarmah D and Khajekar V and Dubey A and Mukherjee U and Kumari A and Bhattacharya S},
title = {Experimental techniques to evaluate IA-MSCs-mediated alleviation of vasogenic edema following ischemic stroke.},
journal = {Methods in enzymology},
year = {2026},
doi = {10.1016/bs.mie.2026.06.021},
note = {PMID: 42624567},
}
TY - JOUR AU - Datta A AU - Malik N AU - Karmarkar G AU - Barik A AU - Sarmah D AU - Khajekar V AU - Dubey A AU - Mukherjee U AU - Kumari A AU - Bhattacharya S TI - Experimental techniques to evaluate IA-MSCs-mediated alleviation of vasogenic edema following ischemic stroke. T2 - Methods in enzymology PY - 2026 DO - 10.1016/bs.mie.2026.06.021 AN - PMID:42624567 ER -
Ischemic stroke induced cerebral edema is a devastating complication which leads to excessive swelling, increased intracranial pressure (ICP) and rapid neurological deterioration. The mortality rate of post-stroke cerebral edema is approximately 80 %. However, therapeutic options for post-stroke cerebral edema are sparce, emphasizing an urgent need to explore new therapeutic strategies for post-stroke edema. In past few decades stem cell therapy has been a promising therapeutic strategy in decreasing infarct volume and promoting functional recovery by various mechanisms following stroke. Previous studies from our lab demonstrated that IA-MSCs administration were beneficial in mitigating post-stroke edema by regulating PKCδ/ MMP9/AQP4 axis and promoting blood-brain-barrier (BBB) integrity. It has also been reported that IA-MSCs administration mitigate post-stroke edema by maintaining BBB integrity, mitochondria function and regulating AQP4 expression by modulation of the SIRT-1/PKCδ/NF-κB axis. In this chapter, we illustrated a multi-level methodological approach to assess therapeutic outcomes of IA-MSCs in alleviating post-stroke cerebral edema by utilizing behavioural analyses, histological staining, biochemical assays, mitochondrial functional assays and molecular investigations by RT-PCR, western blotting and immunofluorescence staining.