An Optimized Protocol for Guidewire-Induced Aortic Valve Stenosis Model in Mice.
Y, Z., J, Z., Y, X., S, L., F, H., J, Z., & M, C. (2026). An Optimized Protocol for Guidewire-Induced Aortic Valve Stenosis Model in Mice.. Journal of visualized experiments : JoVE. https://doi.org/10.3791/72218
Y Z, J Z, Y X, S L, F H, J Z, et al. An Optimized Protocol for Guidewire-Induced Aortic Valve Stenosis Model in Mice.. Journal of visualized experiments : JoVE. 2026; doi: 10.3791/72218
Y Z, J Z, Y X, et al. An Optimized Protocol for Guidewire-Induced Aortic Valve Stenosis Model in Mice.[J]. Journal of visualized experiments : JoVE. 2026. DOI: 10.3791/72218.
@article{y2026,
author = {Zhang Y and Zhou J and Xu Y and Liu S and Huang F and Zhang J and Chen M},
title = {An Optimized Protocol for Guidewire-Induced Aortic Valve Stenosis Model in Mice.},
journal = {Journal of visualized experiments : JoVE},
year = {2026},
doi = {10.3791/72218},
note = {PMID: 42631633},
}
TY - JOUR AU - Zhang Y AU - Zhou J AU - Xu Y AU - Liu S AU - Huang F AU - Zhang J AU - Chen M TI - An Optimized Protocol for Guidewire-Induced Aortic Valve Stenosis Model in Mice. T2 - Journal of visualized experiments : JoVE PY - 2026 DO - 10.3791/72218 AN - PMID:42631633 ER -
Aortic valve stenosis (AVS) is a major cardiovascular disease with increasing prevalence in aging populations, yet the scarcity of diseased valve tissue severely limits mechanistic studies and therapeutic development. This protocol establishes and optimizes a reproducible mouse model of AVS by improving upon the Honda guidewire-induced valve injury method through controlled hemorrhage management. Male and female C57BL/6 mice undergo guidewire-induced aortic valve injury; the procedure is refined by a custom 3D-printed vascular support platform that achieves precise hemorrhage control during guidewire insertion and withdrawal. Outcome measures include 24 h survival, echocardiographic assessment at 4 weeks, and histopathological validation. The optimized protocol achieves a 95% 24 h survival rate (19 of 20 animals); among survivors, 94.7% (18 of 19) meet the predefined hemodynamic success criterion at 4 weeks, yielding an overall model success rate of 90% (18 of 20 animals), confirmed by echocardiography and histological analysis. This standardized model provides a reliable, reproducible platform for investigating mechanisms of valve degeneration and evaluating candidate therapeutic interventions.