Bovine serum albumin sensitizes the mouse brain to lipopolysaccharides.
Y, J., Y, F., X, L., B, Z., Z, Y., R, W., & F, Y. (2026). Bovine serum albumin sensitizes the mouse brain to lipopolysaccharides.. PloS one. https://doi.org/10.1371/journal.pone.0356781
Y J, Y F, X L, B Z, Z Y, R W, et al. Bovine serum albumin sensitizes the mouse brain to lipopolysaccharides.. PloS one. 2026; doi: 10.1371/journal.pone.0356781
Y J, Y F, X L, et al. Bovine serum albumin sensitizes the mouse brain to lipopolysaccharides.[J]. PloS one. 2026. DOI: 10.1371/journal.pone.0356781.
@article{y2026,
author = {Jiang Y and Feng Y and Li X and Zhao B and Yang Z and Wei R and Yuan F},
title = {Bovine serum albumin sensitizes the mouse brain to lipopolysaccharides.},
journal = {PloS one},
year = {2026},
doi = {10.1371/journal.pone.0356781},
note = {PMID: 42647552},
}
TY - JOUR AU - Jiang Y AU - Feng Y AU - Li X AU - Zhao B AU - Yang Z AU - Wei R AU - Yuan F TI - Bovine serum albumin sensitizes the mouse brain to lipopolysaccharides. T2 - PloS one PY - 2026 DO - 10.1371/journal.pone.0356781 AN - PMID:42647552 ER -
This study examined the role of bovine serum albumin (BSA) in lipopolysaccharide (LPS)-induced brain injury. Mice were intraperitoneally injected with either LPS or LPS coupled with BSA. Brain tissues were analyzed for autofluorescence, vascular cell permeability, blood-brain barrier integrity, and pyroptosis. Co-immunoprecipitation and immunostaining were performed to assess the interaction between albumin and LPS. The results showed that, compared to the 5 mg/kg LPS group, the 5 mg/kg BSA-LPS group exhibited enhanced autofluorescence and an increased number of cells with co-localization of propidium iodide (PI) with cluster of differentiation 31 (CD31) and CD13. However, no significant differences were observed between the 25 mg/kg LPS and 25 mg/kg BSA-LPS groups. Horseradish peroxidase (HRP) labeling revealed higher HRP leakage in the 5 mg/kg BSA-LPS group compared to the 5 mg/kg LPS group, a difference that disappeared at the 25 mg/kg dose. Peroxidase staining showed a similar trend. Immunostaining for gasdermin D and CD11b showed significant differences only between the 5 mg/kg LPS and 5 mg/kg BSA-LPS groups. CD68 immunostaining exhibited a similar trend. Furthermore, the study confirmed that albumin binds to LPS in the brain, with more abundant Lipid A+ signals detected in the 5 mg/kg BSA-LPS group compared to the 5 mg/kg LPS group. These findings collectively suggest that albumin boosts the effect of LPS in the brain, probably by binding with LPS and increasing its entry into the brain parenchyma.